RESUMEN
We previously reported preliminary characterization of adipose tissue (AT) dysfunction through the adiponectin/leptin ratio (ALR) and fasting/postprandial (F/P) gene expression in subcutaneous (SQ) adipose tissue (AT) biopsies obtained from participants in the GEMM study, a precision medicine research project. Here we present integrative data replication of previous findings from an increased number of GEMM symptom-free (SF) adults (N = 124) to improve characterization of early biomarkers for cardiovascular (CV)/immunometabolic risk in SF adults with AT dysfunction. We achieved this goal by taking advantage of the rich set of GEMM F/P 5 h time course data and three tissue samples collected at the same time and frequency on each adult participant (F/P blood, biopsies of SQAT and skeletal muscle (SKM)). We classified them with the presence/absence of AT dysfunction: low (<1) or high (>1) ALR. We also examined the presence of metabolically healthy (MH)/unhealthy (MUH) individuals through low-grade chronic subclinical inflammation (high sensitivity C-reactive protein (hsCRP)), whole body insulin sensitivity (Matsuda Index) and Metabolic Syndrome criteria in people with/without AT dysfunction. Molecular data directly measured from three tissues in a subset of participants allowed fine-scale multi-OMIC profiling of individual postprandial responses (RNA-seq in SKM and SQAT, miRNA from plasma exosomes and shotgun lipidomics in blood). Dynamic postprandial immunometabolic molecular endophenotypes were obtained to move towards a personalized, patient-defined medicine. This study offers an example of integrative translational research, which applies bench-to-bedside research to clinical medicine. Our F/P study design has the potential to characterize CV/immunometabolic early risk detection in support of precision medicine and discovery in SF individuals.
RESUMEN
RESUMEN La relevancia que tiene la medición de la fuerza de agarre (FA) se ha incrementado en los últimos años como marcador del estado nutricional, por su relación con la morbilidad y mortalidad y por su creciente aplicación clínica y epidemiológica. Estudios de FA en adultos jóvenes sanos, revelan que se asocia positivamente con variables antropométricas y de composición corporal, mismas que se vincularían con la promoción de la salud en esta población. El objetivo de este estudio fue determinar la correlación entre la FA con variables de antropometría, composición corporal medida por bioimpedancia eléctrica y con la realización de ejercicio, para evaluar su utilidad como indicador del estado nutricional en jóvenes universitarias. Los resultados mostraron que en las estudiantes universitarias la FA se correlacionó positiva y significativamente con variables antropométricas (talla), y de composición corporal (masa muscular). Los profesionales de la salud requieren promover entre este grupo de población la reducción de los factores de riesgo como la baja fuerza muscular a través de la promoción de un estilo de vida activo y en específico de la realización de ejercicio físico de fuerza.
ABSTRACT The relevance of measuring handgrip strength (HGS) has increased in recent years as a marker of nutritional status, due to its relationship with morbidity and mortality and its increasing clinical and epidemiological application. HGS studies in healthy young adults reveal that it is positively associated with anthropometric and body composition variables, the same indicators used for health promotion among young university students. The objective of this study was to determine the correlation between the HGS with anthropometry variables, body composition measured by electrical bioimpedance and with exercise performance, to evaluate its usefulness as an indicator of nutritional status in college students. The results showed that the HGS of female college students was positively and significantly correlated with anthropometric variables (height), and body composition (muscle mass). Among this population group, health professionals need to promote the reduction of risk factors such as low muscle strength through the promotion of an active lifestyle and, specifically, strength exercises.
Asunto(s)
Adolescente , Adulto , Composición Corporal , Antropometría , Estado Nutricional , Ejercicio Físico , Salud , Factores de RiesgoRESUMEN
Interactions between macrophages and adipocytes are early molecular factors influencing adipose tissue (AT) dysfunction, resulting in high leptin, low adiponectin circulating levels and low-grade metaflammation, leading to insulin resistance (IR) with increased cardiovascular risk. We report the characterization of AT dysfunction through measurements of the adiponectin/leptin ratio (ALR), the adipo-insulin resistance index (Adipo-IRi), fasting/postprandial (F/P) immunometabolic phenotyping and direct F/P differential gene expression in AT biopsies obtained from symptom-free adults from the GEMM family study. AT dysfunction was evaluated through associations of the ALR with F/P insulin-glucose axis, lipid-lipoprotein metabolism, and inflammatory markers. A relevant pattern of negative associations between decreased ALR and markers of systemic low-grade metaflammation, HOMA, and postprandial cardiovascular risk hyperinsulinemic, triglyceride and GLP-1 curves was found. We also analysed their plasma non-coding microRNAs and shotgun lipidomics profiles finding trends that may reflect a pattern of adipose tissue dysfunction in the fed and fasted state. Direct gene differential expression data showed initial patterns of AT molecular signatures of key immunometabolic genes involved in AT expansion, angiogenic remodelling and immune cell migration. These data reinforce the central, early role of AT dysfunction at the molecular and systemic level in the pathogenesis of IR and immunometabolic disorders.
